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| Registros recuperados: 29 | |
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| Cavallazzi,José Renato P.; Kasuya,Catarina M.; Soares,Marcos A.. |
| Laccases are enzymes involved in lignin degradation and are produced by various organisms. Due to their low substrate specificity their potential to be used in biotechnological applications has received attention. The addition of laccase inducers to the culture medium of microorganisms can enhance laccase production and facilitate its purification and utilization. The aim of this study was to investigate the effect of some compounds as laccase inducers in cultures of Lentinula edodes (shiitake). First, it was selected a culture medium suitable for laccase production by shiitake using two levels of N (2.6 and 26 mM) and seven levels of Cu (0, 50, 100, 150, 200, 250 and 300 µM). The medium with 2.6 mM N and 250 µM Cu was found to provide the highest laccase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Shiitake; Laccase inducers. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400015 |
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| Diez J,M. Cristina; Gallardo A,Felipe; Saavedra,Gisselle; Cea L,Mara; Gianfreda,Liliana; Alvear Z,Marisol. |
| The activities of laccase, Mn-peroxidase, phosphatase and dehydrogenase were studied in a Chilean Andisol (Temuco Serie) contaminated with pentachlorophenol (PCP). Experiments were conducted on the top of 0-20 cm soil, adding different concentrations of PCP (0.41, 4.1 and 41 mg kg-1). Enzymatic activities and residual concentrations of PCP were evaluated in soil incubated at 25 ± 1 ºC for 112 days. Enzyme activities of PCP-treated soil varied according to the PCP concentration added. For instance, an inhibitory effect on Mn-peroxidase activity was not observed during incubation at the three levels of PCP, whereas acid phosphatase activity was only affected by the 0.41 mg kg-1 PCP treatment. By contrast, laccase and dehydrogenase activities were negatively... |
| Tipo: Journal article |
Palavras-chave: Pentachlorophenol; Andisol; Laccase; Mn-peroxidase; Phosphatase; Dehydrogenase. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912006000300004 |
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| Saparrat,Mario C. N.; Arambarri,Angélica M.; Balatti,Pedro A.. |
| Minimidochium parvum LPSC # 548, a fungus isolated from litter floating on waters of Río Santiago (Provincia de Buenos Aires, Argentina) polluted with industrial effluents and crude-oil, was grown as a shaking culture on a C-limited medium to evaluate its ability to produce extracellular laccase. The effect of anthracene, CuSO4 · 5H2O, ethanol, guaiacol, humic acids, Kraft lignin, MnSO4· H2O, Tween 20 and veratryl alcohol on its growth and extracellular laccase activity levels was also analyzed. The cultures grown on basal medium produced maximum biomass (over 420 mg/100 ml) and maximum extracellular laccase activity (351.7 ±53.3 pkat/ml) after 5 days of incubation. Among the different factors tested, only the humic acids at 0.1 % (w/v) were found to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth; Laccase; Minimidochium parvum. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000100006 |
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| Levin,L.; Ramos,A .M.; Parisi,M .; Gally,M.. |
| Colletotrichum truncatum is the most common pathogen fungus associated with soybean anthracnose. Although the lignin-degrading enzyme laccase has been implicated in pathogenicity of a wide range of plant pathogenic fungi, its biological role in the Colletotrichum -soybean disease system is unknown. The extent of the infection in our country led us to examine laccase production in Argentinean Colletotrichum strains isolated from diseased soybean plants from different geographic locations. Ten strains (eight of them identified as C. truncatum) , were screened for in vitro laccase production. Only six of the isolates, all of them C. truncatum , produced laccase activity when cultured on a defined medium based on pectin and asparagine as carbon and nitrogen... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthracnose; Colletotrichum; Laccase; SDS-PAGE; Soybean. |
| Ano: 2007 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722007000100010 |
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| Yamanaka,Renata; Soares,Clarissa F.; Matheus,Dácio R.; Machado,Kátia M.G.. |
| The expression of the enzymatic system produced by basidiomycetous fungi, which is involved in the degradation of xenobiotics, mainly depends on culture conditions, especially of the culture medium composition. Trametes villosa is a strain with a proven biotechnological potential for the degradation of organochlorine compounds and for the decolorization of textile dyes. The influence of glucose concentration, addition of a vegetable oil-surfactant emulsion, nature of the surfactant and the presence of manganese and copper on the growth, pH and production of laccase, total peroxidase and manganese-dependent peroxidase activities were evaluated. In general, acidification of the medium was observed, with the pH reaching a value close to 3.5 within the first... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Metals; MnP; Vegetable oil; Surfactants. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100019 |
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| Serna C,Liliana; Cuesta A,Fernando L. |
| Contamination is defined as the presence of pollution agents in an ecosystem reaching an abnormal concentration level and causing adverse effects. Due to the important environmental problems that these cause in the food chain, diverse methods have been formulated to minimize those effects; among these enzymatic methods associated to microorganisms or other biological agents are used, and systems that allow to metabolize pollutants in an effective and practical way. The combined use of these systems appears to be the most appropriated option to eliminate pollution agents at a reasonable energy cost which allows their use in the industry. The present review identifies the adequate process conditions for technological implementation of biofilm-type membrane... |
| Tipo: Journal article |
Palavras-chave: Laccase; Biofilm; Membrane system; Halogen compound. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400010 |
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| Singh,Gursharan; Batish,Mona; Sharma,Prince; Capalash,Neena. |
| Various genotoxic textile dyes, xenobiotics, substrates (10 µM) and agrochemicals (100 µg/ml) were tested for enhancement of alkalophilic laccase activity inγ-proteobacterium JB. Neutral Red, Indigo Carmine, Naphthol Base Bordears and Sulphast Ruby dyes increased the activity by 3.7, 2.7, 2.6 and 2.3 fold respectively. Xenobiotics/substrates like p-toluidine, 8-hydroxyquinoline and anthracine increased it by 3.4, 2.8 and 2.3 fold respectively. Atrazine and trycyclozole pesticides enhanced the activityby 1.95 and 1.5 fold respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Γ-proteobacterium; Laccase; Xenobiotics; Dyes; Agrochemicals. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100004 |
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| Gomes,Eleni; Aguiar,Ana Paula; Carvalho,Caio César; Bonfá,Maricy Raquel B.; Silva,Roberto da; Boscolo,Mauricio. |
| Wood rotting Basidiomycetes collected in the "Estação Ecológica do Noroeste Paulista", São José do Rio Preto, São Paulo State, Brazil, concerning Aphyllophorales order and identified as Coriolopsis byrsina SXS16, Lentinus strigellus SXS355, Lentinus sp SXS48, Picnoporus sanguineus SXS 43 and Phellinus rimosus SXS47 were tested for ligninases production by solid state fermentation (SSF) using wheat bran or rice straw as culture media. C. byrsina produced the highest laccase (200 U mL-1) and Lentinus sp produced the highest activities of manganese peroxidase (MnP) and lignin peroxidase (LiP) (7 and 8 U mL-1, respectively), when cultivated on wheat bran. The effect of N addition on enzyme production was studied in medium containing rice straw and the data... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Manganese peroxidase; Lignin peroxidase; Basidiomycetes; Decolorization; Synthetic dye. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100005 |
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| Maciel,Márcia Jaqueline Mendonça; Silva,Ademir Castro e; Ribeiro,Helena Camarão Telles. |
| Ligninolytic enzymes of the basidiomycetes play a crucial role in the global carbon cycle. The demand for application of ligninolytic enzymes complexes of white-rot fungi in industry and biotechnology is ever increasing due to their use in a variety of processes. Ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. This ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds and dyes. Their capacities to remove xenobiotic substances and produce polymeric products make them a useful tool for bioremediation purposes. This paper reviews the applications of ligninolytic... |
| Tipo: Journal article |
Palavras-chave: Laccase; Lignin peroxidase; Manganese peroxidase; White-rot fungi. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600014 |
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| Mikiashvili,Nona A; Isikhuemhen,Omoanghe S; Ohimain,Elijah I. |
| Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Grifola frondosa; Exopolysaccharide; Laccase; Lignin degradation; Peroxidase. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300031 |
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| Spezzia Mazzocco, Teresita. |
| Quince cepas de hongos comestibles pertenecientes a los géneros Pleurotus, Ganoderma, Lentinula y Coprinopsis, fueron evaluadas en cuanto a su actividad enzimática utilizando el medio de cultivo agar con extracto de malta (EMA), adicionado con ácido 2,2'–azino–bis–(3–etillbenzotiazolin–6–sulfónico) (ABTS), 2,6-dimetoxyphenol (DMP) y guayacol. Se seleccionaron nueve cepas para determinar cuantitativamente la actividad de lacasas. Esta evaluación se realizó con el medio de cultivo de extracto de malta líquido (EMB) suplementado con 0.1 mM de sulfato de cobre. Se demostró que las cepas CP-50 de Pleurotus ostreatus y CP-267 de P. pulmonarius son altamente productoras de actividad de lacasas, obteniéndose 4,000 U/ml y 2,500 U/ml, respectivamente. Estas cepas... |
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Palavras-chave: Antraceno; Biorremediación; Hongos comestibles; Lacasas; EDAR; Estrategias para el Desarrollo Agrícola Regional; Doctorado; Anthracene; Bioremediation; Laccase; Mushrooms. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1826 |
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| Luna-acosta, Andrea; Renault, Tristan; Thomas-guyon, Helene; Faury, Nicole; Saulnier, Denis; Budzinski, Helene; Le Menach, K.; Pardon, P.; Fruitier-arnaudin, I.; Bustamante, Paco. |
| In the context of massive summer mortality events of the Pacific oyster Crassostrea gigas, the aim of this study was to investigate the early effects on genes, enzymes and haemocyte parameters implicated in immune defence mechanisms in C. gigas oysters exposed to a potentially hostile environment, i.e. to an herbicide alone or within a mixture. Following 2 h of exposure to the herbicide diuron at 1 mu g L-1 the repression of different genes implicated in immune defence mechanisms in the haemocytes and the inhibition of enzyme activities, such as laccase-type phenoloxidase (PO) in the plasma, were observed. The inhibition of superoxide dismutase (SOD) activity in the plasma was also observed after 6 and 24 h of exposure. In the mixture with the herbicides... |
| Tipo: Text |
Palavras-chave: Bivalve; Pollution; Gene expression; Laccase; Superoxide dismutase; Phagocytosis. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00083/19435/17475.pdf |
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| Registros recuperados: 29 | |
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